Examples

Review development examples that show how NAM testing can be used to compare a NAM with in vivo biology, tune an assay for a specific endpoint, or validate predictions before a project moves forward.

Current example areas include:

• in vivo assessment for a kidney-on-a-chip model, including filtration, clearance, reabsorption, ammoniagenesis, toxicity, and gene expression.
• in chemico optimization for a Direct Peptide Reactivity Assay (DPRA) used to assess skin sensitization.
• in silico model evaluation for a QSAR model predicting long-term sexually dimorphic liver toxicity.
• comparative studies that benchmark tumor organoid screening against patient-derived xenograft models.

These examples illustrate how UC Davis can design targeted in vivo studies to test relevance, predictive value, and translational confidence across different NAM formats.